Journal: Integrative biology : quantitative biosciences from nano to macro

Article Title: Stiffness of pancreatic cancer cells is associated with increased invasive potential

doi: 10.1039/c6ib00135a

Figure Lengend Snippet: (A) Time series of images showing scratch wound invasion of pancreatic ductal cells through Matrigel. Wound confluence is the percentage of wound area covered by cells. Color legend: green is the wound area, blue shows wound confluence in the wound area, and grey represents the confluent cells outside of the wound area. Scale, 300 μm. (B) Schematic illustration showing the modified scratch wound assay. Cells are plated on a layer of thin Matrigel and invade into the thick 3D matrix of overlaid Matrigel that fills the scratch wound. The line plot shows quantification of wound confluence over time. The dotted line indicates the 72 h time point, which we use to compare wound confluence values for statistical significance. The bar plot represents wound confluence at the 72 h time point. Pairwise p-values are determined by a Student’s t-test. *p < 0.05. (C) Schematic illustration showing the transwell migration assay. Cells migrate through the 8 μm pores of a polycarbonate membrane in response to a chemoattractant on the opposite side of the membrane. Images of transwell migration assays showing DRAQ5-labeled nuclei of cells that migrate through the 8 μm pores of a polycarbonate membrane after 12 hours. Scale, 50 μm. Pairwise p-values are determined by a Student’s t-test. *p < 0.05. All error bars represent standard errors. The significance of pairwise comparisons between cell lines is shown in panels B and C by the initial(s) of the cell lines that are significantly different where H: HPDE, Hs: Hs766T, M: MIA PaCa-2, and P: PANC-1. For example, in panel B, HPDE is significantly different (*p < 0.05) from Hs766T (Hs) and PANC-1 (P).

Article Snippet: To image the nucleus, cells are stained with DRAQ5 (1:250 in 0.3% Triton X-100 in 1x PBS; ThermoFisher) at room temperature for 20 minutes.

Techniques: Modification, Scratch Wound Assay Assay, Transwell Migration Assay, Migration, Labeling

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of left ventricular tissue from a 2-day-old newborn. The ConA expression (green channel, A1 ) shows affinity for the cellular membrane of the LV. Nuclei are stained with DRAQ5 (red channel, A2 ). Merged images are shown for all samples ( A3 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Expressing, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of left ventricular tissue from cats in control group. ( A ) Comparison of BS expression (green channel, A1 ) with WGA expression (blue channel, A2 ) in a 6-month-old kitten. The BS expression is nearly absent, while WGA shows preferential binding to the cellular membrane. ( B ) Comparison of Tomato expression (green channel, B1 ) with ConA expression (blue channel, B2 ) in a 7-year-old mature adult. The Tomato shows strong membrane localization, whereas ConA displays cytoplasmic affinity. Sporadic lipofuscin (asterisk *) accumulation is observed in the cytoplasm and is visible across all channels ( B1 – B4 ). ( C ) Comparison of Tomato expression (green channel, C1 ) with ConA expression (blue channel, C2 ) in a 14-year-old senior cat. The Tomato shows strong affinity for both the cellular membrane and vascular wall, while ConA displays more pronounced cytoplasmic localization ( B2 ). Marked lipofuscin (asterisk *) accumulation is present in the cytoplasm, visible across all channels ( C1 – C4 ). Nuclei are stained with DRAQ5 (red channel; A3 , B3 , C3 ). Merged images are shown for all samples ( A4 , B4 , C4 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Control, Comparison, Expressing, Binding Assay, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of right ventricular tissue from a 2-day-old newborn. The ConA expression (green channel; A1 ) shows affinity for the cellular membrane of the RV. Nuclei are stained with DRAQ5 (red channel; A2 ). Merged images are shown for all samples ( A3 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Expressing, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of the right ventricular tissue from cats. ( A ) Comparison of Tomato expression (green channel, A1 ) with ConA expression (blue channel, A2 ) in a 6-month-old kitten. Both lectins exhibit strong membrane localization. ( B ) Comparison of BS expression (green channel, B1 ) with WGA expression (blue channel, B2 ) in a 3-year-old young adult with HCM, where both lectins show moderate cytoplasmic affinity. Nuclei are stained with DRAQ5 (red channel; A3 , B3 ). Merged images are shown for all samples ( A4 , B4 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Comparison, Expressing, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of atrial tissue from a 2-day-old newborn. The ConA expression (green channel; A1 ) shows affinity for both the cellular membrane and cytoplasm of the AT. Nuclei are stained with DRAQ5 (red channel; A2 ). Merged images are shown for all samples ( A3 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Expressing, Membrane, Staining

Journal: Life

Article Title: Glyco-Architectural Remodelling of the Feline Heart: Age- and HCM-Related Insights from Lectin Histochemistry

doi: 10.3390/life16010020

Figure Lengend Snippet: Confocal microscopy micrographs of atrial tissue from cats. ( A ) Comparison of Tomato (green channel, A1 ) and ConA (blue channel, A2 ) expression in a 4-year-old young adult with HCM. Both lectins bind to the cellular membrane, with Tomato exhibiting more intense binding. ( B ) Comparison of Tomato (green channel, B1 ) and ConA (blue channel, B2 ) expression in a 5-year-old young adult with HCM. Both lectins show affinity for both the cellular membrane and cytoplasm, with stronger membrane localization. ( C ) Comparison of BS expression (green channel, C1 ) and WGA expression (blue channel, C2 ) in a 12.5-year-old senior cat. The BS shows low membrane affinity and higher binding to red blood cells (asterisk *) ( C1 , C4 ), while WGA demonstrates strong membrane affinity and moderate red blood cells (asterisk *) and cytoplasmic localization ( C2 , C4 ). Sporadic lipofuscin accumulation is present in the cytoplasm, visible across WGA and Draq5 channels as high intensity points ( C2 , C3 ) and can be observed as magenta points ( C4 ). Nuclei are stained with DRAQ5 (red channel; A3 , B3 , C3 ). Merged images are shown for all samples ( A4 , B4 , C4 ). Images were acquired using a 63×/1.4 Oil Plan Apochromat objective.

Article Snippet: The kits containing Concanavalin A, Wheat Germ Agglutinin and Ricinus communis Agglutinin (Rhodamine Lectin Kit I catalog no. RLK-2200), and Lycopersicon esculentum Lectin, Griffonia (Bandeiraea) simplicifolia Lectin I (Fluorescein Lectin Kit catalog no. FLK-4100) were purchased from Vector Laboratories, and Blue pseudo color (Draq5) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA).

Techniques: Confocal Microscopy, Comparison, Expressing, Membrane, Binding Assay, Staining

Journal: RSC Advances

Article Title: Utilization of metabolic energy in treatment of ocular surface disorders: polyphosphate as an energy source for corneal epithelial cell proliferation

doi: 10.1039/c9ra04409d

Figure Lengend Snippet: Immunofluorescence and ESEM analysis of the outgrown cells, 3 d after initiating of the assays. In the first series the cell layers outgrown from the explants (ex) were stained with (A–C) DRAQ5 to identify the nuclei or double-stained with (D–F) rhodamine/phalloidin and DRAQ5 to express the complex multilayered arrangement of the cells migrated out from the explants; immunofluorescence light microscopy. The rim of the explant is highlighted (ex). In (G–I) higher magnification images of the epithelial cells showing the different degrees of differentiation of the cells; ESEM. In the controls (G) the cells are not continuously attached to each other with cell-to-cell junctions. In addition, a partial exfoliation of the cells is apparent. In contrast, cells migrated from the explants which had been incubated with hp-lysate (HPL) (H), or with polyP (I) smoothly attach to each other and form a homogeneous margin rim of microvilli on their cell surfaces. Surface areas with extensive microvilli decorations are marked (mic).

Article Snippet: Cell outgrowth and differentiation were analyzed after staining with DRAQ5 (nuclei) and rhodamine phalloidin (cytoskeleton), as well as by environmental scanning electron microscopy (ESEM).

Techniques: Immunofluorescence, Staining, Light Microscopy, Incubation